Imaging Core Life Sciences North

 

Tony at workZeiss scopeSuperresolution image

Overview of Services

Full-Service Biological Transmission Electron Microscopy (TEM)


Users drop off fixed samples and we process them for TEM, section the blocks, and provide the user with digital images taken at various magnifications. We can provide 1 micron thick plastic sections suitable for light microscopy, in addition to the 90nm thin sections used for TEM. If users want to review their own samples, we will teach you how to use our TEM. The facility has a research-grade microwave for rapid infiltration of samples, which can also be used for antigen retrieval. Dr. Day has experience with immuno-EM and will provide protocols and ultrathin cryo sections on request.  

 

Optical Microscopy


Two train-to-use instruments are featured. The fully motorized, computer controlled Leica DMI6000 inverted microscope can be used to take brightfield, DIC (20x, 40x only), and polarized light images with a color camera, or these same imaging modes, as well as four channels of fluorescence, using a greyscale camera. The second instrument is a superresolution optical microscope, the Zeiss Elyra S.1. This structured illumination microscope (SIM) can be used to image fluorescence markers at twice the resolving power of confocal or deconvolution microscopes.

Leadership

 

 Brooke Massani, PhD  Tony Day, PhD

 Doug Cromey, MS

 

Brooke Beam Massani, PhD

Operations Director
Phone: (520) 621-3395
Email: bbeam@email.arizona.edu

 

William “Tony” Day, PhD

Manager, Biological TEM
Phone: (520) 626-6009
Email: waday@email.arizona.edu

 

Doug Cromey, MS

Manager, Optical Microscopy
Phone: (520) 626-2824
Email: dcromey@email.arizona.edu

Hours of Operation and Location

Hours

Location

Monday - Friday   
8:00AM - 5:00PM


24/7 Access to Trained Users   

Life Sciences North
Room 410

Links and Resources

  1. See us on the Microscopy Alliance Website
  2. See us on the RDI Imaging Cores Website
  3. Sample preparation guidance to enable achieving superresolution is available on this webpage.
  4. The Imaging Core Life Sciences North Equipment Kiosk.

 

Contacts

Name Role Phone Email Location
Imaging Cores Life Science North
General Facility Contact
 
520-626-6009
 
ImagingLSN@email.arizona.edu
 
Life Sciences North, Rm# 410
 
Core Facility Business Office
Billing Inquiries
 
520-621-4064
 
CoreBusiness@email.arizona.edu
 
Gould Simpson 10th Floor
 

Available Equipment and Resources

Name Price
FEI Tecnai Spirit Transmission Electron Microscope  
Zeiss Elyra S.1  
Leica DMI6000  
Olympus IMT-2 Image Analysis Workstation  

Map


LIFE SCIENCES NORTH at the UA Health Sciences Center, 1501 N. Campbell Ave, Tucson, AZ, 85724


The ground floor entrances to this building all require previously authorized CatCard access. During
regular work hours the building can be easily accessed via the bridge connecting the building to the
College of Medicine. A floor plan map (opens in a new tab) is available.

 

 

NOTE: building access after hours is only available through the use of a previously authorized CatCard.


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