Imaging Core Life Sciences North

Overview of Services

Full-Service Biological Transmission Electron Microscopy (TEM)

Users drop off fixed samples and we process them for TEM, section the blocks, and provide the user with digital images taken at various magnifications. We can provide 1 micron thick plastic sections suitable for light microscopy, in addition to the 90nm thin sections used for TEM. If users want to review their own samples, we will teach you how to use our TEM. The facility has a research-grade microwave for rapid infiltration of samples, which can also be used for antigen retrieval. Dr. Tonino has experience with immuno-EM and will provide protocols and ultrathin cryo sections on request.  

Optical Microscopy

Two train-to-use instruments are featured. The fully motorized, computer controlled Leica DMI6000 inverted microscope can be used to take brightfield, DIC (20x, 40x only), and polarized light images with a color camera, or these same imaging modes, as well as four channels of fluorescence, using a greyscale camera. The second instrument is a superresolution optical microscope, the Zeiss Elyra S.1. This structured illumination microscope (SIM) can be used to image fluorescence markers at twice the resolving power of confocal or deconvolution microscopes.

Leadership

Paula Tonino, PhD

Manager, Electron Microscopy
Phone: (520) 626-2670
Email: toninop@arizona.edu

Binh Chau, BS

Assistant TEM Scientist
Phone: (520) 626-6009
Email: bchau@arizona.edu

Doug Cromey, MS

Manager, Optical Microscopy
Phone: (520) 626-2824
Email: dcromey@email.arizona.edu

Hours

Location

Monday - Friday   
8:00AM - 5:00PM

24/7 Access to Trained Users   

Life Sciences North
Room 410