Imaging Core Life Sciences North

 

Tony at workZeiss scopeSuperresolution image

Overview of Services

Full-Service Biological Transmission Electron Microscopy (TEM)


Users drop off fixed samples and we process them for TEM, section the blocks, and provide the user with digital images taken at various magnifications. We can provide 1 micron thick plastic sections suitable for light microscopy, in addition to the 90nm thin sections used for TEM. If users want to review their own samples, we will teach you how to use our TEM. The facility has a research-grade microwave for rapid infiltration of samples, which can also be used for antigen retrieval. Dr. Tonino has experience with immuno-EM and will provide protocols and ultrathin cryo sections on request.  

 

Optical Microscopy


Two train-to-use instruments are featured. The fully motorized, computer controlled Leica DMI6000 inverted microscope can be used to take brightfield, DIC (20x, 40x only), and polarized light images with a color camera, or these same imaging modes, as well as four channels of fluorescence, using a greyscale camera. The second instrument is a superresolution optical microscope, the Zeiss Elyra S.1. This structured illumination microscope (SIM) can be used to image fluorescence markers at twice the resolving power of confocal or deconvolution microscopes.

Leadership

 

 Dr. Paula Tonino

 

 Binh Chau

 Doug Cromey, MS

Paula Tonino, PhD

Manager, Electron Microscopy
Phone: (520) 626-2670
Email: toninop@arizona.edu

Binh Chau, BS

Assistant TEM Scientist
Phone: (520) 626-6009
Email: bchau@arizona.edu

Doug Cromey, MS

Manager, Optical Microscopy
Phone: (520) 626-2824
Email: dcromey@email.arizona.edu

Hours of Operation and Location

Hours

Location

Monday - Friday   
8:00AM - 5:00PM


24/7 Access to Trained Users   

Life Sciences North
Room 410

Links and Resources

  1. See us on the Microscopy Alliance Website
  2. See us on the RDI Imaging Cores Website
  3. Sample preparation guidance to enable achieving superresolution is available on this webpage.
  4. The Imaging Core Life Sciences North Equipment Kiosk.

 

Contacts

Name Role Phone Email Location
Imaging Cores Life Science North
General Facility Contact
 
520-626-6009
 
ImagingLSN@email.arizona.edu
 
Life Sciences North, Rm# 410
 
Core Facility Business Office
Billing Inquiries
 
520-621-4064
 
CoreBusiness@email.arizona.edu
 
Keating Room 200
 

Available Equipment and Resources

Name Price
FEI Tecnai Spirit Transmission Electron Microscope  
Reichert-Jung Ultracut E Microtome  
Leica Ultracut UCT Microtome  
Zeiss Elyra S.1  
Unassisted Use Sun-Sat 08:00 AM - 06:00 PM
Internal $48.00 /hr
For-Profit $75.00 /hr
Training Mon-Fri 08:00 AM - 05:00 PM
Internal $98.00 /hr
For-Profit $152.50 /hr
Assisted Use Mon-Fri 08:00 AM - 05:00 PM
Internal $98.00 /hr
For-Profit $152.50 /hr
Leica DMI6000  
Image Analysis Workstation  

Map


LIFE SCIENCES NORTH at the UA Health Sciences Center, 1501 N. Campbell Ave, Tucson, AZ, 85724


The ground floor entrances to this building all require previously authorized CatCard access. During
regular work hours the building can be easily accessed via the bridge connecting the building to the
College of Medicine. A floor plan map (opens in a new tab) is available.

 

 

NOTE: building access after hours is only available through the use of a previously authorized CatCard.