Phenotyping Core Facility (UAPC)

About Phenotyping Core

The objective of the University of Arizona Phenotyping Core (UAPC) is to provide resources to investigate phenotypes of genetically altered mice and small animal models of disease. The UAPC offers investigators full-service support of in vivo cardiovascular and muscle physiology studies in normal and diseased states through surgical models, imaging techniques, and functional physiological assessments. In order to offer the most thorough analysis of mouse physiology, we utilize state-of-the-art facilities and equipment, including:

• Visualsonics Vevo 2100 echocardiography system
• Scisense pressure-volume admittance catheters for cardiac catheterization and pressure-volume analysis
• Metabolic treadmill system, voluntary running cages, and swimming chambers for exercise studies
• Whole body Plethysmography for respiration studies
• Mouse muscle test system to study muscle function and mechanics including foot plate mechanics, Rotorod, Grip Strength, and Digigait.
• Intact muscle mechanics (Aurora Scientific)
• DSI blood pressure and biopotential telemetry system
• ECG (Ecgene mouse specific)
• Intact cardiomyocyte contractility and Ca2+ transient studies (Cytocypher, Ionoptix inc)
• Engineered Heart Tissue System
• Isolated Heart system
• Microsurgical operating room
• Modalities for assessment of disease states including ischemia/reperfusion injury, myocardial infarction, volume overload, and pressure overload

Physiological parameters, particularly cardiovascular parameters, in mice can be greatly influenced by differences in strain, sex, stress state, and manner of handling, which can lead to a high degree of variability in data acquisition. In addition, surgical models of disease in small animals and methods of evaluating physiological parameters are technically challenging. Relying on the experience and expertise of a core facility offers the best opportunity for obtaining consistent and quality physiologic data. The UAPC serves to fill this important role at the University of Arizona by providing investigators access to state-of-the-art equipment, high-end facilities, and experienced staff. Furthermore, as a core service and facility resource the availability of the UAPC can be utilized to strengthen grant applications.

For information on specific services not listed here or to discuss specialized needs contact us.

The UAPC welcomes both University of Arizona investigators as well as outside investigators/institutions.

UAPC Mission

The Mission of the UAPC is to provide expertise and consolidated resources for investigations of small animal phenotypes. In order to support this mission, the UAPC provides investigators:

1. Validated phenotyping techniques, which allow for consistent models and results with little experimental variation.
2. Consultation on experimental design, protocols, and data analysis.
3. Customized services tailored to meet individual investigators' needs.
4. Assistance with IACUC protocols, manuscript preparation, and grant writing.
5. Training of research staff in phenotyping techniques.

Leadership

Location and hours of operation

Links and Resources

1. https://phenocore.ahsc.arizona.edu/

Contacts