Viral Production Core

Overview of Services

The University of Arizona Viral Production Core facility offers services for the generation of high-titer AAV, Adenoviral and Lentiviral particles. We provide a full cloning service for the generation of plasmids of interest, a high quality purification of viral particles and analysis of specific viral properties. Recombinant viruses are powerful tools that can be used to overexpress, silence, or edit genes. Exceptional features of Adeno-Associated Virus (AAV) allow for generating effective disease models and studying unique gene functions and genotype-phenotype relationships in various animal models. Lentivirus vectors used for the in vitro transduction of nearly all cell types, including difficult-to-transfect cells. Adenoviruses are the most effective and promising tool for gene therapy.

Leadership

Henk Granzier, PhD, Professor and Core Director

Olga Alekhina, PhD, Research Specialist

Links and Resources

https://viralcore.cmm.arizona.edu/

Contacts

Name	Role	Phone	Email	Location
Olga Alekhina, PhD	Research Specialist	(520) 626-4198	oalekhina@arizona.edu	College of Medicine - Tucson, Medical Research Building, 3rd Floor
Henk Granzier, PhD	Director	(520) 626-3641	granzier@arizona.edu	College of Medicine - Tucson, Medical Research Building, 3rd Floor

Service list

Name	Description	Price
Adeno-Associated Virus (AAV) ultra-purified midi scale (>1E13 GC/ml, 500 ul)	AAV particles are purified using iodixanol gradient ultracentrifugation; AAV titer is 1E13 GC/ml in 500 ul sample. The estimated timeline for this process is approximately 3-4 weeks, which may vary depending on the number of jobs in queue ahead of yours.	Internal $1,638.00 each
Adeno-Associated Virus (AAV) ultra-purified mini scale (>1E13GC/ml, 250 ul)	AAV particles are purified using iodixanol gradient ultracentrifugation, AAV titer is 1E13GC/ml in a 250 ul sample. The estimated timeline for this process approximately 3-4 weeks, which may vary depending on the number of jobs in queue ahead of yours.	Internal $1,084.00 each
Adenovirus primary stock (recombination and amplification)	Adenovirus recombination and amplification. Preparation of adenovirus primary stock. The estimated timeline for this process is approximately 3-4 weeks, which may vary depending. on the number of jobs in queue ahead of yours.	Internal $955.00 each
Adenovirus ultra-purified maxi scale (1E9 PFU/ml, 1000 ul)	Adenovirus particles are purified using CsCl gradient ultracentrifugation. Titer is 1E9 PFU/ml in a 500 ul sample. The estimated timeline for this process is aproximately 3-4 weeks, which may vary depending on the number of jobs queue ahead of yours.	Internal $1,719.00 each
Adenovirus ultra-purified midi scale (1E9 PFU/ml, 500 ul)	Adenovirus particles are purified using CsCl gradient ultracentrifugation. Titer is 1E9 PFU/ml in a 500 ul sample. The estimated timeline for this process is aproximately 3-4 weeks, which may vary depending on the number of jobs queue ahead of yours.	Internal $1,151.00 each
Lentivirus non-ultra purified for cell culture (Crude lysate)	For in vitro experiments, you will be provided with a crude lentivirus lysate. The estimated timeline for this process is approximately 2 weeks, which may vary on the number of jobs queue ahead of yours.	Internal $532.00 each
Lentivirus ultra-purified large-scale packaging	Lentivirus particles are purified using sucrose gradient ultracentrifugation. Titer is 1E9 IFU/ml, in a 250 ul sample. These purified lentivirus particles are suitable both for in vitro and in vivo applications. The estimated timeline for this process is approximately 3 weeks, which may vary on the number of jobs queue ahead of yours.	Internal $1,152.00 each
Single-step cloning service 1	The process involves excision and insertion of gene of interest into the destination plasmid, followed by midiprep DNA extraction. The customer provides the gene of interest DNA (plasmid). The estimated timeline for this process is aproximately 2 weeks, which may vary depending on the number of jobs queue ahead of yours.	Internal $662.00 each
Single-step cloning service 2	The process involves excision and insertion of gene of interest into the destination plasmid, followed by midiprep DNA extraction. The customer provides the gene of interest (cDNA). The estimated timeline for this process is aproximately 3-4 weeks, which may vary depending on the number of jobs queue ahead of yours.	Internal $1,159.00 each

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► AAV packaging (2)
Adeno-Associated Virus (AAV) ultra-purified mini scale (>1E13GC/ml, 250 ul)	AAV particles are purified using iodixanol gradient ultracentrifugation, AAV titer is 1E13GC/ml in a 250 ul sample. The estimated timeline for this process approximately 3-4 weeks, which may vary depending on the number of jobs in queue ahead of yours.	Internal $1,084.00 each
Adeno-Associated Virus (AAV) ultra-purified midi scale (>1E13 GC/ml, 500 ul)	AAV particles are purified using iodixanol gradient ultracentrifugation; AAV titer is 1E13 GC/ml in 500 ul sample. The estimated timeline for this process is approximately 3-4 weeks, which may vary depending on the number of jobs in queue ahead of yours.	Internal $1,638.00 each
▼ ► Adenovirus packaging (3)
Name	Description	Price
Adenovirus ultra-purified midi scale (1E9 PFU/ml, 500 ul)	Adenovirus particles are purified using CsCl gradient ultracentrifugation. Titer is 1E9 PFU/ml in a 500 ul sample. The estimated timeline for this process is aproximately 3-4 weeks, which may vary depending on the number of jobs queue ahead of yours.	Internal $1,151.00 each
Adenovirus ultra-purified maxi scale (1E9 PFU/ml, 1000 ul)	Adenovirus particles are purified using CsCl gradient ultracentrifugation. Titer is 1E9 PFU/ml in a 500 ul sample. The estimated timeline for this process is aproximately 3-4 weeks, which may vary depending on the number of jobs queue ahead of yours.	Internal $1,719.00 each
Adenovirus primary stock (recombination and amplification)	Adenovirus recombination and amplification. Preparation of adenovirus primary stock. The estimated timeline for this process is approximately 3-4 weeks, which may vary depending. on the number of jobs in queue ahead of yours.	Internal $955.00 each
▼ ► Lentivirus packaging (2)
Name	Description	Price
Lentivirus non-ultra purified for cell culture (Crude lysate)	For in vitro experiments, you will be provided with a crude lentivirus lysate. The estimated timeline for this process is approximately 2 weeks, which may vary on the number of jobs queue ahead of yours.	Internal $532.00 each
Lentivirus ultra-purified large-scale packaging	Lentivirus particles are purified using sucrose gradient ultracentrifugation. Titer is 1E9 IFU/ml, in a 250 ul sample. These purified lentivirus particles are suitable both for in vitro and in vivo applications. The estimated timeline for this process is approximately 3 weeks, which may vary on the number of jobs queue ahead of yours.	Internal $1,152.00 each
▼ ► Cloning service (2)
Name	Description	Price
Single-step cloning service 1	The process involves excision and insertion of gene of interest into the destination plasmid, followed by midiprep DNA extraction. The customer provides the gene of interest DNA (plasmid). The estimated timeline for this process is aproximately 2 weeks, which may vary depending on the number of jobs queue ahead of yours.	Internal $662.00 each
Single-step cloning service 2	The process involves excision and insertion of gene of interest into the destination plasmid, followed by midiprep DNA extraction. The customer provides the gene of interest (cDNA). The estimated timeline for this process is aproximately 3-4 weeks, which may vary depending on the number of jobs queue ahead of yours.	Internal $1,159.00 each

Available Equipment and Resources

Name		Price