Welcome!
The University of Arizona Viral Production Core facility offers services for the generation of high-titer AAV, Adenoviral and Lentiviral particles. We provide a full cloning service for the generation of plasmids of interest, a high quality purification of viral particles and analysis of specific viral properties. Recombinant viruses are powerful tools that can be used to overexpress, silence, or edit genes. Exceptional features of Adeno-Associated Virus (AAV) allow for generating effective disease models and studying unique gene functions and genotype-phenotype relationships in various animal models. Lentivirus vectors used for the in vitro transduction of nearly all cell types, including difficult-to-transfect cells. Adenoviruses are the most effective and promising tool for gene therapy.
Henk Granzier, PhD, Professor and Core Director
Olga Alekhina, PhD, Research Specialist
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Olga Alekhina, PhD |
Research Specialist
|
(520) 626-4198
|
oalekhina@arizona.edu
|
College of Medicine - Tucson, Medical Research Building, 3rd Floor
|
| Henk Granzier, PhD |
Director
|
(520) 626-3641
|
granzier@arizona.edu
|
College of Medicine - Tucson, Medical Research Building, 3rd Floor
|
| Service list |
| ► AAV packaging (2) | |||
| Name | Description | Price | |
|---|---|---|---|
| Adeno-Associated Virus large-scale packaging |
AAV particles purified with iodixanol gradient ultracentrifugation. We can guarantee AAV titer as 1E13GC/ml in 250 ul. Estimated timeline is 3-4 weeks. The timeline depends on how many jobs are ahead of yours.
|
Internal
$835.00
each
|
|
| Adeno-Associated Virus small-scale packaging |
AAV crude lysate for in vitro experiments. Titer: 1E12 GC/mL, 250 uL. Estimated timeline is 2-3 weeks. The timeline depends on how many jobs are ahead of yours. |
Internal
$400.00
each
|
|
| ► Adenovirus packaging (3) | |||
| Name | Description | Price | |
| Adenovirus amplification and purification |
Large-scale purified Adenovirus packaging. We guarantee 1E9 PFU/ml in 250 ul. Estimated timeline is 4-5 weeks. The timeline depends on how many jobs are ahead of yours.
|
Internal
$840.00
each
|
|
| Adenovirus primary stock, amplification only |
Small-scale adenovirus packaging, amplification only. Titer is 1E8 PFU/mL, 2000 uL. Estimated timeline is 3-4 weeks. The timeline depends on how many jobs are ahead of yours. |
Internal
$430.00
each
|
|
| Pre-made Adenoviral controls |
Please see our list of current inventory of purified Pre-Made Control viral particles at https://viralcore.cmm.arizona.edu/pre-made-adenovirus-controls Titer: 1E9 PFU/mL, 150 uL. |
Internal
$255.00
each
|
|
| ► Cloning service (2) | |||
| Name | Description | Price | |
| Complex cloning service |
More complex cloning strategies. Estimated timeline is 1-2 months. The timeline depends on how many jobs are ahead of yours |
Internal
$1,390.00
each
|
|
| Single-step cloning service |
Excision and insertion of gene of interest into the destination plasmid, midiprep DNA purification. Estimated timeline 2 weeks. The timeline depends on how many jobs are ahead of yours. |
Internal
$875.00
each
|
|
| ► Lentivirus packaging (2) | |||
| Name | Description | Price | |
| Lentivirus large-scale packaging |
Lentivirus particles purified with sucrose gradient centrifugation. We guarantee titer as 1E9 IFU/mL in 200 uL. Estimated timeline is 3-4 weeks. The timeline depends on how many jobs are ahead of yours. |
Internal
$800.00
each
|
|
| Lentivirus small-scale packaging |
Crude lentivirus lysate for in vitro experiments. Titer: 2E8 IFU/mL, 100 uL-custom. Estimated timeline is 2-3 weeks. The timeline depends on how many jobs are ahead of yours |
Internal
$415.00
each
|
|
| Name | Price | |
|---|---|---|
