Microbiome Core at Steele Children’s Research Center

Overview of Services

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To request a quote or initiate a service please use the Request Services tab above 

 

The Microbiome Core at Steele Children’s Research Center, University of Arizona, was established to provide end-to-end technical and research support to study microbial communities' structure and function. Our Core supports investigators in creating questions and hypothesss related to microbiome study, provide study design input as needed in areas including but not limited to:
- sample collection
- microbial genomic DNA extraction
- number of samples required to provide a sufficient statistical power
- selection of appropriate sequencing method (16S amplicon profiling or metagenome sequencing).

The core primarily serves investigators at the University of Arizona, but also other academic institutions and industry with interest in microbiome analysis including human microbiome and microbiome in animal models of human disease, as well as all environmental samples (e.g. soil, water).

The goal of the Microbiome Core is to stimulate research of the relationship between a host organism and its microbiome in both health and disease states. Its mission is to provide the research community with the facility and expertise to characterize complex microbial communities and microbial interactions. The Core has state of the art instrumentation and extensively trained personnel that provide support from experimental design to data analysis.

 

Overview of Services (selected):


Microbial DNA Extraction
• Fecal Samples
• Oral, skin, vaginal swabs
• Saliva samples
• Provide Pre-extracted DNA for Processing


16S Sequencing
• The Core has adopted the protocol (16S, ITS) from Earth Microbiome Project
• Up to 450 unique barcodes
• Target 515F-806R V4 region of the 16S rRNA gene
• Pair-ended (2x150) sequencing on MiSeq Illumina platform


Metagenomic Sequencing
• The Core uses Qiagen and Illumina library preparation kits
• Metagenome sequencing on NextSeq 550/550 Illumina

 

Leadership

Daniel Laubitz

Core Director

(520) 626-1401

microbiome@email.arizona.edu

Pawel Kiela

Professor

(520) 626-5170

pkiela@peds.arizona.edu

Leisa Grijalva

Financial Manager

(520) 626-6956

grijalva@peds.arizona.edu

Nic Propp

Accountant

(520) 626-5176

ndpropp@peds.arizona.edu

 

Location and hours of operation

 Hours  Location

 9am - 5pm
 Monday - Friday  

 1501 N Campbell Ave 
 Steele Children's Research Center 
 Tucson, AZ 85724


Links and Resources

Microbiome Core Website

Need help?

(520) 626-1401

microbiome@email.arizona.edu

Billing Questions?

(520) 626-5170

grijalva@peds.arizona.edu

 

Contacts

Name Role Phone Email Location
Daniel Laubitz
Core Director
 
(520) 626-1401
 
microbiome@email.arizona.edu
 
Steele Children's Research Center
 
Leisa Grijalva
Financial Manager
 
(520) 626-6956
 
grijalva@peds.arizona.edu
 
Biomedical Research Lab
 

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