PANDA Core for Genomics and Microbiome Research at Steele Children’s Research Center

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The PANDA Core for Genomics and Microbiome Research (formerly Microbiome Core) at the Steele Children’s Research Center, University of Arizona, was established to provide end-to-end technical and research support to study microbial communities' structure and function. Our Core supports investigators in creating questions and hypothesis related to microbiome study, provide study design input as needed in areas including but not limited to:
- sample collection
- microbial genomic DNA extraction
- number of samples required to provide a sufficient statistical power
- selection of appropriate sequencing method (16S amplicon profiling or metagenome sequencing)

The core primarily serves investigators at the University of Arizona, but also other academic institutions and industry with interest in microbiome analysis including human microbiome and microbiome in animal models of human disease, as well as all environmental samples (e.g. soil, water).

The goal of the PANDA Core is to stimulate research of the relationship between a host organism and its microbiome in both health and disease states. Its mission is to provide the research community with the facility and expertise to characterize complex microbial communities and microbial interactions. The Core has state of the art instrumentation and extensively trained personnel that provide support from experimental design to data analysis.

 

Overview of Services (selected):

Microbial DNA Extraction
• Fecal Samples
• Oral, skin, vaginal swabs
• Saliva samples
• Provide Pre-extracted DNA for Processing

16S Sequencing
• The Core has adopted the protocol (16S, ITS) from Earth Microbiome Project
• Up to 450 unique barcodes
• Target 515F-806R V4 region of the 16S rRNA gene
• Pair-ended (2x150) sequencing on MiSeq Illumina platform

• Pair-ended (2x150) sequencing on NextSeq 1000/2000 Illumina platform

Metagenomic Sequencing
• The Core uses Qiagen, Illumina, QuantaBio library preparation kits
• Metagenome sequencing on Illumina NextSeq 1000/2000 and NovaSeq 6000

RNA Sequencing
• total transcryptome, total RNA with rRNA depletion
• mRNA, RNA sequencing with mRNA enrichment

 9am - 5pm
 Monday - Friday  

 1501 N Campbell Ave 
 Steele Children's Research Center 
 Tucson, AZ 85724

Need help?

(520) 626-1401

microbiome@arizona.edu

Billing Questions?

(520) 626-5170

grijalva@peds.arizona.edu