Quantitative Proteomics Laboratory

Lab Heading Picture

Overview of Services

The mission of The Quantitative Proteomics Lab is to provide collaborators the opportunity to take advantage of next generation mass spectrometers and software platforms to perform label-free quantitative proteomics.  The lab is centered around two dueling Thermo Scientific Fusion Lumos tribrid mass spectrometers, each coupled to a Thermo Scientific Ultimate 3000 RSnanoLC system and a Thermo Scientific Fisher EASY-SPRAY Source and C18 column. Our data analysis software platform consists of Mascot, Scaffold, and Progenesis. We offer space within the Proteomics Lab to learn how to prepare samples, as well as a touchdown desk outfitted with an Alienware laptop for data analysis. 

 

We are here to assist collaborators in the design, execution, and analysis of quantitative proteomics studies using a collaborative and immersive approach. It is our goal to not only deliver conclusive results from quantitative proteomic projects but to also teach the principles and concepts of mass spectrometry and quantitative proteomics so that collaborators can better understand the science of the process. You do not need any prior proteomics experience - it is our responsibility to help you design, execute, and understand every step of the method. We also offer the opportunity for trainees to get hands-on bench experience in proteomic sample prep if that is something collaborators are interested in.

 

We have four specialties that we perform, using a “label-free” format. “Label free” means that the quantitative proteomics is performed on cells that are not labeled, so no extra steps are required to label the cells and perform the quantification. Collaborators simply lyse and go.

 

Interactomes

 

INTERACTOMES

 

Identifying potential protein interaction partners through characterizing protein interactomes.

 

PTM

 

PTM

 

Identifying post-translational modifications (PTMs) and quantifying changes in PTM abundance after cell treatments (like kinase inhibitors, for example), across animal models of disease states or human control versus disease state tissue.

 

Proteomes

 

PROTEOMES

 

Profiling protein abundance changes across entire proteomes, from either cell culture systems, animal models, or human studies. Since we have some amazing instruments, we are able to get pretty solid depth of proteome coverage that is competitive in the current field of proteomics.

 

Subcellular fraction proteomes

 

SUBCELLUAR FRACTION PROTEOMES

 

Profiling protein abundance changes across subcellular fractions, from either cell culture systems, animal models, or human studies.

Leadership 

Lawrence Mandarino

Paul Langlais Natalie Barker
Professor, Medicine
Chief, Division of Endocrinology
Director, UA Center for Disparities in Diabetes, Obesity and Metabolism
(520) 626-8367
Associate Professor, Medicine
Director, Proteomics Lab 
UA Center for Disparities in Diabetes, Obesity and Metabolism
Division of Endocrinology, College of Medicine
(520) 626-1342
Research Specialist, Senior of Langlais Lab
Assistant Director of Proteomics Lab
UA Center for Disparities in Diabetes, Obesity and Metabolism
Division of Endocrinology, College of Medicine
520-626-1342

Location and hours of operation

Hours Location

Monday - Friday

8:00 AM - 5:00 PM

 

College of Medicine

1501 N Campbell Ave

Room 5111

Tucson, AZ 85724

Links and Resources

Visit The Proteomics Lab on the web here.

Contacts

Name Role Phone Email Location
Paul Langlais
Facility Director
 
520-626-1342
 
langlais@email.arizona.edu
 
College of Medicine Room 5111
 

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