iLab Organizer :: Analytical and Biological Mass Spectrometry

Pipetting The Analytical & Biological Mass Spectrometry Core Facility offers mass measurements of synthetic products, polymers, and whole proteins. The Core is located in room 124J in the BIO5 Keating Bioresearch Building on the North Campus. Facility personnel determine the mass of the molecular components of a sample for structural confirmation and/or identification. Services include confirmation or determination of unknowns, Proteomics, and, in the future, Metabolomics and Lipidomics.

The Core is equipped with mass spectrometers that can measure molecular masses with high mass accuracy, including a Bruker 9.4 Tesla FT-ICR, a Thermo LTQ Orbitrap Velos, and a Thermo Q Exactive Plus Orbitrap. The latter two instruments are dedicated to peptide analysis. The Core also has a second Q Exactive Plus Orbitrap that will be dedicated to the analysis of lipids and metabolites. Mass measurements of whole proteins and polymers can be made using the Core’s Bruker Autoflex Speed MALDI-TOF. Routine analyses (low mass accuracy) of a variety of compounds are made using the Bruker AmaZon SL Ion Trap, the ABI Sciex 4000 Q-Trap and the Shimadzu GC-MS. Additional equipment in the Core includes a UV-HPLC with a fraction collector, an AKTA FPLC for protein separations, and 1D- and 2D-gel electrophoresis equipment.

Personnel


Krishna Parsawar, PhD Manager Email: krishnaparsawar@arizona.edu Phone: (520) 626-4161	Cynthia David, PhD Assistant Staff Scientist Email: cdavid@email.arizona.edu Phone: (520) 626-4173	Yelena Feinstein, MS Research Specialist Email: feinstei@pharmacy.arizona.edu Phone: (520) 626-4161	Kristen Kallen-Keck, BS Analytical Research Coordinator Email: keck@email.arizona.edu Phone: (520) 626-4161

Hours of Operation and Locations

Hours

Locations

Monday - Friday

9:00 AM – 5:00 PM

24/7 Access for Trained Users

Keating Bioresearch Building, Room 124J

1657 E Helen Street

Tucson, AZ 85721

(520) 626-4161

Samples may be dropped off at the UAGC freezer in the entryway to Room 106

Links and Resources

TBA

Contacts

Name	Role	Phone	Email	Location
Analytical & Biological Mass Spec	General Facility Contact - Keating	520-626-4161	MassSpec@email.arizona.edu	Keating Bioresearch Building, Room 106
Core Facility Business Office	Billing Inquiries	520-621-4064	CoreBusiness@email.arizona.edu	Keating Bioresearch Building, 2nd Floor

Available Equipment and Resources

Name		Price
BIACORE T200
Michrom HPLC
AmaZon Ion Trap
solariX 2XR FTMS
Autoflex Speed MALDI TOF
GC
Shimadzu HPLC
LTQ Orbitrap Velos
Q Exactive Plus - Proteomics
Q Exactive Plus - Metabolomics
Orbitrap Exploris 480
Isoelectric Focuser
Laser Capillary Puller
SDS PAGE
Metabolomics_Data_Processing_Computer

Map

</div> </div> <br /> </div> </div> <div class="ui custom_network tab" data-tab=arizona-core-network id="custom_prt_ntw"></div> <script> //<![CDATA[ var _gaq = _gaq || []; _gaq.push(['_setAccount', 'UA-145723597-1']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); //]]> </script> </div> <div id="footer">  © Agilent Technologies, Inc. 2022 <a name='bottom'></a> </div> </div> <script type="text/javascript"> </script> <script type="text/javascript"> setTimeout(function(){var a=document.createElement("script"); var b=document.getElementsByTagName("script")[0]; a.src=document.location.protocol+"//dnn506yrbagrg.cloudfront.net/pages/scripts/0018/5029.js?"+Math.floor(new Date().getTime()/3600000); a.async=true;a.type="text/javascript";b.parentNode.insertBefore(a,b)}, 1); </script> </body> </html>