Analytical and Biological Mass Spectrometry

Overview of Services

The Analytical & Biological Mass Spectrometry Core is located in room 124J in the BIO5 Keating Bioresearch Building on the North Campus. Facility personnel determine the mass of the molecular components of a sample for structural confirmation and/or identification. Services include confirmation of synthetic products, measurement of polymers and whole proteins, assistance with determination of unknowns, Proteomics, and, in the future, Metabolomics and Lipidomics.

The Core is equipped with mass spectrometers that can measure molecular masses with high mass accuracy, including a Bruker 9.4 Tesla FT-ICR, a Thermo LTQ Orbitrap Velos, and a Thermo Q Exactive Plus Orbitrap. The latter two instruments are dedicated to peptide analysis. The Core also has a second Q Exactive Plus Orbitrap that will be dedicated to the analysis of lipids and metabolites.  Mass measurements of whole proteins and polymers can be made using the Core’s Bruker Autoflex Speed MALDI-TOF. Routine analyses (low mass accuracy) of a variety of compounds are made using the Bruker AmaZon SL Ion Trap, the ABI Sciex 4000 Q-Trap and the Shimadzu GC-MS. Additional equipment in the Core includes a UV-HPLC with a fraction collector, an AKTA FPLC for protein separations, and 1D- and 2D-gel electrophoresis equipment.

Personnel

Krishna Parsawar, PhD
Manager
Email: krishnaparsawar@email.arizona.edu
Phone: (520) 626-4161

Cynthia David, PhD
Assistant Staff Scientist
Email: cdavid@email.arizona.edu  
Phone: (520) 626-4173

Yelena Feinstein, MS
Research Specialist
Email: feinstei@pharmacy.arizona.edu
Phone: (520) 626-4161

Kristen Kallen-Keck, BS
Analytical Research Coordinator
Email: keck@email.arizona.edu 
Phone: (520) 626-4161

Hours

Locations

Monday - Friday

9:00 AM – 5:00 PM

24/7 Access for Trained Users

Keating Bioresearch Building, Room 124J

1657 E Helen Street

Tucson, AZ 85721

 (520) 626-4161

Samples may be dropped off at the GATC freezer in the entryway to Room 106