Analytical and Biological Mass Spectrometry

 

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Overview of Services

The Analytical & Biological Mass Spectrometry Core has two locations where researchers can bring samples for analysis. Facility personnel determine the mass of the molecular components of a sample for structural confirmation and/or identification. The Chemistry Building lab focuses on confirmation of synthetic products, measurement of polymers and whole proteins, and assistance with determination of unknowns. In the Keating location, work most often involves Proteomics, and, in the future, Metabolomics and Lipidomics.

 

The Core is equipped with mass spectrometers that can measure molecular masses with high mass accuracy, including a Bruker 9.4 Tesla FT-ICR, a Thermo LTQ Orbitrap Velos, and a Thermo Q Exactive Plus Orbitrap. The latter two instruments are dedicated to peptide analysis. The Core also has a second Q Exactive Plus Orbitrap that will be dedicated to the analysis of lipids and metabolites.  Mass measurements of whole proteins and polymers can be made using the Core’s Bruker Autoflex Speed MALDI-TOF. Routine analyses (low mass accuracy) of a variety of compounds are made using the Bruker AmaZon SL Ion Trap, the ABI Sciex 4000 Q-Trap and the Shimadzu GC-MS. Additional equipment in the Core includes a UV-HPLC with a fraction collector, an AKTA FPLC for protein separations, and 1D- and 2D-gel electrophoresis equipment.

Personnel

 

 

 

 

 

Krishna Parsawar, PhD
Manager

Email: krishnaparsawar@email.arizona.edu

Phone: 

Cynthia David, PhD
Assistant Staff Scientist

Email: cdavid@email.arizona.edu  
Phone: (520) 626-4173

Yelena Feinstein, MS
Research Specialist
Email: feinstei@pharmacy.arizona.edu  

Phone: (520) 626-4161

Kristen Kallen-Keck, BS
Analytical Research Coordinator
Email: keck@pharmacy.arizona.edu  

Phone: (520) 626-9136

Hours of Operation and Locations

Hours
Locations

Monday - Friday

9:00 AM – 5:00 PM

24/7 Access for Trained Users

Keating Bioresearch Building, Room 106

1657 E Helen Street

Tucson, AZ 85721

 (520) 626-4161

Monday - Friday

8:30 AM – 4:30 PM

Chemistry Building, Room 112

1306 E University Blvd

Tucson, AZ 85721

(520) 626-9136

Links and Resources

  1. TBA

Contacts

Name Role Phone Email Location
Analytical & Biological Mass Spec
General Facility Contact - Chemistry
 
520-626-9136
 
MassSpec@email.arizona.edu
 
Chemistry Building Room 112
 
Analytical & Biological Mass Spec
General Facility Contact - Keating
 
520-626-4161
 
MassSpec@email.arizona.edu
 
Keating Bioresearch Building, Room 106
 
Core Facility Business Office
Billing Inquiries
 
520-621-4064
 
CoreBusiness@email.arizona.edu
 
Gould Simpson 10th Floor
 

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