|
Sara Centuori, PhD Shared Resource Director Research Asst. Professor, Medicine Phone: (520) 626-3249 Email: smb4@email.arizona.edu |
Mark Curry Shared Resource Manager Phone: (520) 621-2047 Email: markcurry@email.arizona.edu |
Hours |
Location |
Human Immune Monitoring Facility (HIMF) |
|
Monday - Friday |
Arizona Cancer Center |
Flow Cytometry Shared Resource (FCSR) |
|
Monday - Friday |
Arizona Cancer Center |
Name | Role | Phone | Location | |
---|---|---|---|---|
Flow Cytometry Shared Resource |
General Facility Contact
|
520-621-2047
|
Flow@email.arizona.edu
|
Leon Levy Cancer Center Room 0935
|
Core Facility Business Office |
Billing Inquiries
|
520-621-4064
|
CoreBusiness@email.arizona.edu
|
Keating Room 200
|
Human Immune Monitoring Facility |
Human Immune Monitoring
|
520-626-7254
|
Leon Levy Cancer Center Room 0913
|
Service list |
► Human Immune Monitoring (15) | |||
Name | Description | Price | |
---|---|---|---|
PBMC Isolation from ≤60ml whole blood |
Isolation of PBMCs using Ficoll gradient separation.
Custom separation protocols that match your needs can also be used. |
Internal
$110.00
each
Non-Profit/Educational $168.00 each For-Profit $180.00 each |
|
PBMC Isolation from ≥60ml whole blood |
Isolation of PBMCs using Ficoll gradient separation.
Custom separation protocols that match your needs can also be used. |
Internal
$170.00
each
Non-Profit/Educational $225.00 each For-Profit $280.00 each |
|
PBMC Pellet from CPT Blood Collection Tube |
PBMC purification, pelleting, and snap freezing of PBMCs from whole blood after RBC lysis. |
Inquire | |
Plasma or Serum Extraction |
Isolation and aliquoting of plasma and/or serum |
Internal
$35.00
each
Non-Profit/Educational $47.50 each For-Profit $60.00 each |
|
White Blood Cell Isolation |
Isolation of total white cells using high speed centrifugation. |
Internal
$60.00
each
Non-Profit/Educational $80.00 each For-Profit $100.00 each |
|
Biobanking |
Storage of biological samples in a -20, -80, or -195 degree environment.
This is a one time per sample banking fee. Continual biobanking fees do not apply. |
Internal
$35.00
each
Non-Profit/Educational $47.50 each For-Profit $60.00 each |
|
Nucleic Acid Purification |
Purification of Nucleic Acids using a standardized kit. If a user requests a specific kit not used by HIMF, the user is responsible for providing their kit to HIMF.
High throughput 96-well purification methods also available. Inquire for pricing. |
Internal
$35.00
each
Non-Profit/Educational $47.50 each For-Profit $60.00 each |
|
Tissue Dissociation |
Enzymatic and mechanical dissociation of solid tissue into single cells in preparation for downstream analysis such as flow cytometry. Tissue inputs can include solid tumor as well as a varity of human and murine tissues. Various preservation techniques are available.
Please inquire about your specific project by contacting us through the cosultation form under the reservations tab. |
Internal
$110.00
each
Non-Profit/Educational $145.00 each For-Profit $180.00 each |
|
Sample Staining |
Staining in preparation for Flow Cytometry or other immunologic assays.
Prices are per sample, but can be batched for a discount depending on sample number. Reagents such as staining antibodies and buffers are charged separately. |
Internal
$105.00
each
Non-Profit/Educational $137.50 each For-Profit $170.00 each |
|
Cell Culturing and Maintenance |
Short term maintenance of cell culture utilizing HIMF facilities. This includes cell culturing for assay prep. We can support a variety of tissues and cells. |
Internal
$45.00
/hr
Non-Profit/Educational $60.00 /hr For-Profit $75.00 /hr |
|
ELISA |
Enzyme linked immunosorbent assay on a 96 well plate. Custom and kit based ELISA available. |
Inquire | |
Fixation for CyTOF |
Preperation of samples for Mass Cytometry. |
Internal
$45.00
each
Non-Profit/Educational $60.00 each For-Profit $75.00 each |
|
Seahorse Assay |
Available assays include:
Kits and reagents sold separately. |
Internal
$125.00
each
Non-Profit/Educational $162.50 each For-Profit $200.00 each |
|
Custom Assay |
General catch all for immunologic services not listed. Please contact the facility using our Consultation Form to discuss your project.
Assays can include:
Please contact us directly to discuss how we can support your project. |
Internal
$75.00
/hr
Non-Profit/Educational $97.50 /hr For-Profit $120.00 /hr |
|
HIMF - Staff Labor |
Internal
$35.00
/hr
|
► Agilent Seahorse XFe96 Metabolic Analyzer (1) | ||||||||||||||||||||||||
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► Flow Cytometry (7) | ||||||||||||||||||||||||
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